The second edition of this bestselling book helps researchers, teachers, and students properly understand and use the major techniques and methods employed in cellular and molecular biology today. Each chapter covers the principles behind the various methods and techniques, a detailed step-by-step description of each protocol, and applications of the protocol to different systems. In addition to presenting new protocols in all chapters, Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes in vitro transcription methods, analysis of DNA sequences, and new bioseparation techniques. Several new chapters cover cellular and subcellular localization of proteins, combinatorial chemistry, and data mining.